京都府立医科大学 眼科学教室

Corneal Molecular Biology / Gene Expression and Genetics

研究実績

Publications 1992-1997
( Original and Collaborated Achievements )

Corneal Molecular Biology / Gene Expression and Genetics


























































A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA.

Invest Ophthalmol Vis Sci 37(9):1800‐1809,1996. (Nishida K, Adachi W, Shimizu‐Matsumoto A, Kinoshita S, Mizuno K, Matsubara K, Okubo K)

* The quantitative and qualitative aspects of gene expression in human corneal epithelium are described. The most abundant gene is Apolipoprotein J. The second gene, cornea‐specific human keratin 12, was isolated and its full‐size cDNA was sequenced.

 
Isolation and chromosomal localization of a cornea‐specific human keratin 12 gene and detection of four mutations in Meesmann corneal epithelial dystrophy.

Am J Hum Genet 61:1268‐1275,1997. (Nishida K, Honma Y, Dota A, Kawasaki S, Adachi W, Nakamura T, Quantock AJ, Hosotani H, Yamamoto S, Okada M, Shimomura Y, Kinoshita S)

* The exon‐intron boundary structure and chromosomal localization of human keratin 12 are described. It has 8 exons and 7 introns and is localized in 17q12. This study also demonstrated that keratin 12 mutations cause Meesmann corneal epithelial dystrophy.

 
Apolipoprotein J expression in human ocular surface epithelium.

Invest Ophthalmol Vis Sci 37(11):2285‐2292,1996. (Nishida K, Kawasaki S, Adachi W, Kinoshita S)

* The expression of ApoJ, the most abundant gene in human corneal epithelium, was examined. ApoJ mRNA is detected in all layers of corneal epithelium, prominently in basal cells, and its protein was detected in the apical cell layers. Since ApoJ was detected in non‐keratinized epithelia, but not in keratinized epithelium, ApoJ may act to maintain the tear‐tissue interface.

 
Human corneal and conjunctival epithelia express MUC1 mucin.

Invest Ophthalmol Vis Sci 36(9):1818‐1827,1995. (Inatomi T, Spurr‐Michaud S, Tisdale AS, Gipson IK )

* Corneal and conjunctival epithelia express a membrane type of mucin, MUC1, on their apical surface. This mucin may interact with the secreted mucin expressed by conjunctival goblet cells.

 
Expression of secretory mucin genes by human conjunctival epithelia.

Invest Ophthalmol Vis Sci 37(2):1684‐1692,1996. (Inatomi T, Spurr‐Michaud S, Tisdale A, Zhan Q, Feldman ST, Gipson IK )

* Two conjunctival mucins were identified. MUC5AC mucin is specifically expressed by conjunctival goblet cells and MUC4 mucin is expressed by stratified conjucntival epithelium.

 
Cloning of rat Muc5AC mucin gene : comparison of its structure and tissue distribution to that of human and mouse homologies.

Biochem Biophys Res Commun 236:789‐797,1997. ( Inatomi T, Tisdale A, Zhan Q, Spurr‐Michaud S, Gipson IK )

* Partial sequence of rat Muc5AC mucin gene including tandem repeat and cysteine rich domains were reported.  This gene is specifically expressed by conjunctival goblet cells like those in human.

 
Mucin genes expressed by the ocular surface epithelium.

Prog Retina Eye Res 16(1):81‐98,1997. ( Inatomi T, Gipson IK )

* This review summarizes recent work aimed at determining the molecular character of mucins expressed by the conjunctival and corneal epithelium.

 
Efficient gene transduction by epstein‐barr virus (EBV) ‐based vectors coupled with cationic liposome and HVJ‐liposome.

Biochem Biophys Res Commun 238:795‐ 799,1997. (Satoh E, Osawa M, Tomiyasu K, Hirai H, Shimazaki C, Oda Y, Nakagawa M, Kondo M, Kinoshita S, Mazda O, Imanishi J)